Search

100% Secure delivery without contacting the courier

    In Stock

    Dairy Lactoperoxidase Test Kit

    Order No.: EC0189

    Lactoperoxidase (LPO) is a peroxidase naturally existing in milk. With the presence of hydrogen peroxide, it is capable of oxidizing thiocyanate ions to produce products with broad antimicrobial activity. Lactoperoxidase has an important role in the dairy cattle immune system. Lactoperoxidase is one of the most heat-stable enzymes found in milk. Pasteurization cannot deactivate it completely. Ultra-high-temperature processing will deactivate it completely. Hence, LPO is an internal index for monitoring and evaluating heat process intensity, testing the LPO activity level has been widely used in dairy production industry.

    During the heat treatment of milk processing, the level heat intensity is related to the nutritional value of milk. By testing the LPO activity in milk, the nutritional value in the sample can be determined. High volume LPO indicates the milk kept most of its nutrients.

    Lactoperoxidase Test Kit uses colrimetry, can quickly quantitate the LPO activity level efficiently.

    Principles

    LPO Stop Solution

    LPO Substrate Blue Indicator Yellow Indicator

    The kit is enzyme catalysis based. Add sample or standard to microwell plate, then pipette the Substrate solutions to the wells, the LPO in sample or standard will react with the LPO Substrate and turn blue color. After adding Stop Solution, the blue solution will change to yellow color. Read the absorbance of the wells with Microplate reader using 450nm filter. The LPO activity in the sample is positively correlated with the absorbance.

    Scope of Application

    Quantitative analysis of Lactoperoxidase in raw milk, pasteurized milk, UHT milk.

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm)

    ---- Vortex

    ---- Centrifuge (≥4000rpm)

    ---- Centrifuge tube: 50mL

    ---- Whatman 1# filter paper

    ---- Reagent reservoir (optional)

    ---- Constant Temperature Incubator

    ---- Timer

    ---- Micropipette: Single channel pipette (100-1000μL, 10mL)

    Multi-channel pipette (30-300μL)

    2. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component96 wells
    Microwell plate12 × 8 wells
    1 vial of Standard

    (Black cap, brown vial)

    0.6mL, 12000U/L
    Sample Diluting Buffer

    (White cap)

    12mL
    LPO Substrate

    (Ready-to-use, brown cap)

    12mL
    Concentrated Sample Extraction Buffer A (20×)40mL
    Concentrated Sample Extraction Buffer B (20×)40mL
    1.5mL centrifuge tubes with cap15
    Instruction1
    Plate Cover Sheet2
    Testing Report1

    Reviews

    There are no reviews yet.

    Write a review

    Your email address will not be published.

    Bestsellers

    Aflatoxin B1 Quantitative Rapid Lateral Flow Test Kit

    $144.00
    (0 Reviews)
    The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin B1 and is intended for use in nuts, grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

    (0 Reviews)
    The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Aflatoxin M1 Immunoaffinity Column

    $143.00
    (0 Reviews)
    Order No.: QC0068,QC0069,QC0131,QC0071Column Capacity:100 ngVolumn: 3mLPackage: 25/50T 

    Immunoaffinity Column for 2-in-1 Aflatoxins (B1,B2,G1,G2) and Ochratoxin A

    $375.00
    (0 Reviews)
    The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample  solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.Order No.: HCM3725B/HCM3750BProduct Properties: AFT B1/B2/G1/G2 300ng, OTA 100ngSpecifications: 3mL,25T/50T

    Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

    $143.00
    (0 Reviews)
    The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

    Milk Albendazole Metabolite Rapid Test Kit

    (0 Reviews)
    The Albendazole Metabolite Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of albendazole metabolite antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Aflatoxins B1 ELISA Test Kit for Feed

    $96.00
    (0 Reviews)
    The Aflatoxin B1 ELISA Test Kit is a competitive ELISA for the qualitative or quantitative analysis of aflatoxin B1 in feed and feed material.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

     

    Back to Top
    Product has been added to your cart