Search

100% Secure delivery without contacting the courier

    In Stock

    Qualitative or Quantitative Antibiotic Sulfonamides ELISA Test Kit

    Order No.:ME08CER

    Qualitative or quantitative analysis of sulfonamides residues in animal tissue, eggs, egg powder, swine urine, etc.

    Meizheng Bio-Tech Company Overview-V2022-1 2022.6.14

     

    Summary

    Sulfonamides (SAs) are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.

    HPLC/GC-MS method is now widely used in the detection of sulfonamides residues, but its high cost and complex operation make it unacceptable.

    Use Principles

    Sulfonamides ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The sulfonamides antigen is precoated on the wells. The precoated antigen compete the sulfonamides antibody (antibody solution) with sulfonamides in the sample, anti- sulfonamides antibody binds to the sulfonamides-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the sulfonamides concentration of the samples is derived.

    Cross-reactivity

    Sulfamerazine 100%

    Sulfadiazine 188%

    Sulfamonomethoxine 413%

    Sulfaquinoxaline 44%

    Sulfachlorpyridazine 75%

    Sulfaclozine 68%

    Sulfametoxydiazine 365%

    Sulfamethazine 38%

    Sulfamethoxypyridazine 42%

    Sulfamethazole 33%

    Sulfaethoxypyridazine 35%

    Sulfadoxine 361%

    Sulfadimethoxine 299%

    Sulfisomidine 129%

    Sulfamethizole 140%

    Sulfabenzamide 39%

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Oscillator

    ---- Vortex

    ---- Rubber Bulb Syringe

    ---- Termovap Sample Concentrator

    ---- Homogenizer

    ---- Centrifuge

    ---- Constant Temperature Incubator

    ---- Centrifuge tube: 2mL, 10mL/ 7mL, 50mL

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- Neutral alumina (100-200mesh)

    ---- Ethyl acetate (AR)

    ---- Methanol (AR)

    ---- Acetonitrile (AR)

    ---- N-hexane (AR)

    ---- 18M Concentrated Sulfuric Acid (H2SO4)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate96T
    5 vials of standard1mL each
    High concentration standard (1ppm)1mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated sample diluting buffer I40mL
    Concentrated sample diluting buffer II40mL
    Concentrated sample extracting buffer15 mL
    Sample extractant1
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb and 13.5ppb.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

    Reviews

    There are no reviews yet.

    Write a review

    Your email address will not be published.

    Bestsellers

    Aflatoxin B1 Quantitative Rapid Lateral Flow Test Kit

    (0 Reviews)
    The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin B1 and is intended for use in nuts, grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

    (0 Reviews)
    The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Aflatoxin M1 Immunoaffinity Column

    (0 Reviews)
    Order No.: QC0068,QC0069,QC0131,QC0071Column Capacity:100 ngVolumn: 3mLPackage: 25/50T 

    Immunoaffinity Column for 2-in-1 Aflatoxins (B1,B2,G1,G2) and Ochratoxin A

    (0 Reviews)
    The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample  solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.Order No.: HCM3725B/HCM3750BProduct Properties: AFT B1/B2/G1/G2 300ng, OTA 100ngSpecifications: 3mL,25T/50T

    Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

    (0 Reviews)
    The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

    Milk Albendazole Metabolite Rapid Test Kit

    (0 Reviews)
    The Albendazole Metabolite Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of albendazole metabolite antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Aflatoxins B1 ELISA Test Kit for Feed

    (0 Reviews)
    The Aflatoxin B1 ELISA Test Kit is a competitive ELISA for the qualitative or quantitative analysis of aflatoxin B1 in feed and feed material.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

     

    Back to Top
    Product has been added to your cart