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Dairy Staphylococcus aureus Enterotoxin ELISA Test Kit

It can qualitatively detect the total content of Staphylococcus aureus enterotoxin in raw milk, finished milk, fermented milk, milk beverages, whey powder and milk powder samples.

Flyer-Staphylococcus aureus Enterotoxin ELISA Test Kit. pdf

Brochure-Dairy Test. PDF

Meizheng Bio-Tech Company Overview. PDF

ManufacturerMeizheng
Order No.LR50501
AnalyteStaphylococcus aureus enterotoxin
Sample TypeRaw milk, finished milk, fermented milk, milk beverages, whey powder and milk powder samples.
MethodologyDouble antibody sandwich ELISA method
Packaging96 Tests
StorageT  The storage temperature for the kits is 2-8℃.
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.

 Introduction

  1. Enterotoxin (SE) is a pathogenic substance that causes Staphylococcus aureus food poisoning. According to its antigenicity, it can be divided into 8 serotypes A-E and G-I, of which SEA is the most common. Enterotoxins in food are not easily inactivated during processing and can cause symptoms such as vomiting and diarrhea. In severe cases, they can cause multiple organ dysfunction and be life-threatening.
  2. This kit is a new generation of Staphylococcus aureus total enterotoxin (SET) detection product using ELISA , which can minimize operating errors and work intensity.

Principle

This kit adopts double antibody sandwich ELISA method. Pre-coat the total amount of Staphylococcus aureus enterotoxin antibody on the microplate. Both the total amount of pre-coated enterotoxin antibody and detection antibody bind to the enterotoxin in the sample at the same time. And then the complex combines with the HRP-conjugate to form an antibody- enterotoxin- antibody- enzyme- HRP-conjugate, which can be colored by TMB substrate. The absorbance of the sample is positively correlated with the content of Staphylococcus aureus enterotoxin.

 Parameters

Sensitivity: 0.25-1ppb

Limit of Detection (Coefficient of Variation of the kit is less than 10%):

SampleLOD (ppb)
SEASEBSECSEDSEE
Fresh milk, finished milk, whey powder, milk powder0.50.250.250.251
Fermented milk31.51.51.56
milk beverages10.50.50.52

 Materials Required but Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex

—- Incubation container

—- Refrigerated centrifuge

—- Balance (sensibility: 0.01g)

—- Centrifuge tube: 2mL, 5mL, 7mL, 50mL

—-Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)  Multi-channel pipet (30µL -300µL)

  1. Reagent:

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

—- Sodium hydroxide (1mol/L)

Kit components

Component96 wells
Microwell plate12 test strips of 8 wells each
SET antibody solution12ml
SET Enzyme conjugate12ml
Substrate A6ml
Substrate B6ml
Negative control3ml
Positive control3ml
Concentrated wash buffer (10×)40ml *2
Insert1
Cover sheet2

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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