|Analyte||Staphylococcus aureus enterotoxin|
|Sample Type||Raw milk, finished milk, fermented milk, milk beverages, whey powder and milk powder samples.|
|Methodology||Double antibody sandwich ELISA method|
|Storage||T The storage temperature for the kits is 2-8℃.|
|Shelf life||The validity for this kit is 12 months|
|Lead time||Shipped within 5-12 working days.|
- Enterotoxin (SE) is a pathogenic substance that causes Staphylococcus aureus food poisoning. According to its antigenicity, it can be divided into 8 serotypes A-E and G-I, of which SEA is the most common. Enterotoxins in food are not easily inactivated during processing and can cause symptoms such as vomiting and diarrhea. In severe cases, they can cause multiple organ dysfunction and be life-threatening.
- This kit is a new generation of Staphylococcus aureus total enterotoxin (SET) detection product using ELISA , which can minimize operating errors and work intensity.
This kit adopts double antibody sandwich ELISA method. Pre-coat the total amount of Staphylococcus aureus enterotoxin antibody on the microplate. Both the total amount of pre-coated enterotoxin antibody and detection antibody bind to the enterotoxin in the sample at the same time. And then the complex combines with the HRP-conjugate to form an antibody- enterotoxin- antibody- enzyme- HRP-conjugate, which can be colored by TMB substrate. The absorbance of the sample is positively correlated with the content of Staphylococcus aureus enterotoxin.
Limit of Detection (Coefficient of Variation of the kit is less than 10%):
|Fresh milk, finished milk, whey powder, milk powder||0.5||0.25||0.25||0.25||1|
Materials Required but Not Provided
---- Microwell plate Reader (450nm/630nm)
---- Incubation container
---- Refrigerated centrifuge
---- Balance (sensibility: 0.01g)
---- Centrifuge tube: 2mL, 5mL, 7mL, 50mL
----Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL) Multi-channel pipet (30µL -300µL)
---- Deionized water
---- Concentrated sulfuric acid (18 mol/L)
---- Sodium hydroxide (1mol/L)
|Microwell plate||12 test strips of 8 wells each|
|SET antibody solution||12ml|
|SET Enzyme conjugate||12ml|
|Concentrated wash buffer (10×)||40ml *2|
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.