Background:
Chloramphenicol (CAP) is a widely used antibiotic, but has serious side effects, which can cause aplastic anemia, agranulocytosis and other diseases.
The detection method is sensitive, fast, simple and specific, only need 45minu for operating and a small amount of sampling.
Use Principles:
The Chloramphenicol ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Chloramphenicol in the sample, anti-Chloramphenicol antibody binds to the HRP enzyme conjugate. Then pipe the substrate solution to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Chloramphenicol concentration of the samples is derived.
| Manufacturer | Meizheng |
| Order No. | ME01A |
| Analyte | Chloramphenicol |
| Sample Type | Poultry tissues, feed, swine urine, eggs and etc. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Chloramphenicol: 100% Chloramphenicol Alkali: 0.5% Methylchloramphenicol: ≤0.05% Florfenicol:＜0.01% Flurfenicomide:＜0.1% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 0.02ppb |
| Limit of detection | Poultry Tissue ……………………….. 0.02ppb Egg ………………………………….. 0.04ppb Feed ………………………………….. 0.08ppb Swine Urine ………………………… 0.1ppb |
| Percent Recovery, % | 100±20% |
| Precision (CV %): | Intra-lab assay: CV%≤10% |
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 6 vials of Standard | 1ml each |
| High concentration standard (100ppb) | 1ml each |
| CAP Enzyme Conjugate | 6ml |
| CAP Antibody Solution | 6ml |
| Substrate A | 6ml |
| Substrate B | 6ml |
| Concentrated Wash Buffer (10X) | 40ml |
| Concentrated sample diluting buffer | 40ml |
| Concentrated sample extracting buffer | 30ml |
| IFU | 1 |
| Testing Report | 1 |
Note: The concentrations of the standards are 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb and 1.62ppb respectively.
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Vortex Mixer
—- Constant temperature incubator
—- Centrifuge
—- Balance (sensibility: 0.01g)
—-Water bath
—- Homogenizer
—- Nitrogen evaporator
—- Graduated suction pipet (10ml)
—- Micropipet: Single channel pipet (10µl-100µl, 100µl-1000µl)
Multi-channel pipet (30µl-300µl)
—- Centrifuge tube (50ml, 10ml or 7ml)
- Reagent:
—- Deionized water
—-Ethyl acetate (analytically pure)
—–N-hexane (analytically pure)
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
