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    Honey Qualitative or Quantitative Furaltadone Metabolite ELISA Test Kit

    Qualitative or quantitative analysis of furaltadone metabolite in honey.

    Order No.: SC0044

    Summary 

    Nitrofurans, which are effective against a wide range of bacteria. The metabolites of nitrofuran drugs are furacilin metabolite (SEM), furaltadone metabolite (AMOZ), furazolidone metabolite (AOZ), and furantoin metabolite (AHD), which combined with proteins stably. Therefore, the detection of their metabolites is often used to reflect the residual status of nitrofuran drugs. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

    Use Principles 

    Furaltadone Metabolite (AMOZ) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The AMOZ antigen is precoated on the wells. The precoated antigen compete the AMOZ antibody (antibody solution) with AMOZ in the sample, anti-AMOZ antibody binds to the AMOZ-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the AMOZ concentration of the samples is derived.

    Cross-reactivity

    Furaltadone metabolite (AMOZ)  100%

    Furantoin metabolite (AHD) <0.1%

    Furazolidone metabolite (AOZ) <0.1%

    Furacilin metabolite (SEM) <0.1%

    Materials Required but Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Centrifuge

    ---- Constant Temperature Incubator / Water bath

    ---- Balance (sensibility: 0.01g)

    ---- Termovap Sample Concentrator

    ---- Rubber suction bulb

    ---- Graduated pipette: 10mL

    ---- Centrifuge tube: 7mL / 10mL, 50mL

    ---- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

    Multi-channel pipette (30-300μL)

    1. Reagent:

    ---- Deionized/distilled water

    ---- Ethyl acetate (AR)

    ---- N-hexane (AR)

    ---- Methanol (AR)

    ---- Sodium hydroxide (NaOH) (AR)

    ---- Concentrated hydrochloric acid (HCl) (AR)

    ---- Dipotassium hydrogen phosphate trihydrate (K2HPO4·3H2O) (AR)

    ---- 18M concentrated sulfuric acid (H2SO4)

    Provided Materials and Reagent

    Component 96 wells
    Antigen coated plate12 × 8 wells
    6 vials of standard1mL each
    AHD-AOZ-AMOZ-SEM Mixed High Concentration Standard (100ppb)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Concentrated Derivatization Reagent (10×)1mL
    Package Insert1
    Testing Report1
    Plate cover sheet2

    Note: The concentrations of the standards are 0μg/kg, 0.05μg/kg, 0.15μg/kg, 0.45μg/kg, 1.35μg/kg and 4.05μg/kg.

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