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    Furazolidone ELISA Test Kit for Poultry Tissues, Liver and Eggs

    The Furazolidone (AOZ) ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of Furazolidone (AOZ) in samples of poultry tissues, liver, eggs etc.

    This product is currently out of stock and unavailable.

    Summary

    Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

    Use Principles

    Furazolidone (AOZ) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The AOZ antigen is precoated on the wells. The precoated antigen compete the AOZ antibody (antibody solution) with AOZ in the sample, anti-AOZ antibody binds to the AOZ-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the AOZ concentration of the samples is derived.

    Cross-reactivity

    Furazolidone (AOZ)…………………………………………………100%

    Furantoin (AHD)………………………………………………………<0.1%

    Furaltadone (AMOZ)…………………………………………………<0.1%

    Furacilin (SEM)…………………………………………………………<0.1%

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex mixer

    ---- Centrifuge

    ---- Homogenizer

    ---- Constant temperature incubator

    ---- Balance (sensibility: 0.01g)

    ---- Nitrogen evaporator

    ---- Water bath

    ---- Transfer pipette: 10mL

    ---- Centrifuge tube: 10mL, 50mL,7mL

    ---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Ethyl acetate (AR)

    ---- Deionized water

    ---- N-hexane (AR)

    ---- Methanol (AR)

    ---- Concentrated hydrochloric acid (AR)

    ---- NaOH (AR)

    ---- Dipotassium Hydrogen Phosphate Trihydrate (K2HPO4.3H2O) (AR)

    ---- Concentrated sulfuric acid (18 mol/L)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of standard1mL each
    High concentrated standard (100ppb)1mL
    AOZ Enzyme Conjugate6mL
    AOZ Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated wash buffer (10×)40mL
    Concentrated sample diluting buffer (2X)40mL
    Concentrated derivatization reagent (10X)1mL
    IFU1
    Testing Report1
    Plate cover sheet2

    Note: The concentrations of the standards are 0ppb, 0.03ppb, 0.09ppb, 0.27ppb, 0.81ppb and 2.43ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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