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Honey Qualitative or Quantitative Furantoin Metabolite ELISA Test Kit

Qualitative or quantitative analysis of furantoin metabolite in honey.

Order No.: SC0042


Nitrofurans, which are effective against a wide range of bacteria. The metabolites of nitrofuran drugs are furacilin metabolite (SEM), furaltadone metabolite (AMOZ), furazolidone metabolite (AOZ), and furantoin metabolite (AHD), which combined with proteins stably. Therefore, the detection of their metabolites is often used to reflect the residual status of nitrofuran drugs. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

Use Principles 

Furantoin Metabolite (AHD) ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The AHD antigen is precoated on the wells. The precoated antigen compete the AHD antibody (antibody solution) with AHD in the sample, anti-AHD antibody binds to the AHD-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the AHD concentration of the samples is derived.


Furantoin metabolite (AHD) 100%

Furaltadone metabolite (AMOZ) <0.1%

Furazolidone metabolite (AOZ) <0.1%

Furacilin metabolite (SEM) <0.1%

Materials Required but Not Provided

  1. Devices:

—- Microwell plate reader (450nm/630nm)

—- Oscillator / Vortex

—- Centrifuge

—- Constant Temperature Incubator / Water bath

—- Termovap Sample Concentrator

—- Balance (sensibility: 0.01g)

—- Rubber suction bulb

—- Graduated pipette: 10mL

—- Centrifuge tube: 7mL / 10mL, 50mL

—- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

Multi-channel pipette (30-300μL)

  1. Reagent:

—- Deionized/distilled water

—- Ethyl acetate (AR)

—- N-hexane (AR)

—- Methanol (AR)

—- Sodium hydroxide (NaOH) (AR)

—- Concentrated hydrochloric acid (HCl) (AR)

—- Dipotassium hydrogen phosphate trihydrate (K2HPO4·3H2O) (AR)

—- 18M concentrated sulfuric acid (H2SO4)

Provided Materials and Reagent

Component 96 wells
Antigen coated plate12 × 8 wells
6 vials of standard1mL each
AHD-AOZ-AMOZ-SEM Mixed High Concentration Standard (100ppb)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Concentrated Derivatization Reagent (10×)1mL
Package Insert1
Testing Report1
Plate cover sheet2

Note: The concentrations of the standards are 0μg/kg, 0.05μg/kg, 0.15μg/kg, 0.45μg/kg 1.35μg/kg and 4.05μg/kg.


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