Immunoaffinity Column for Folic Acid IAC Column

The immunoaffinity column can selectively adsorb folic acid from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.

Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio and improve the accuracy of the detection method.

Order No.: QC0135

Product Properties: 500ng

Specifications: 3mL,25T

Matrix: Milk powder,Dairy products

Overview:

Folic acid is an important type of B vitamin that is normally found in foods. It helps your body produce and maintain new cells, and also helps prevent changes to DNA that may lead to cancer. As a medication, folic acid is used to treat folic acid deficiency and certain types of anemia (lack of red blood cells) caused by folic acid deficiency.

1. Principle:

The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and folic acid in the sample is extracted, filtered, and diluted, and then passed slowly through the folic acid immunoaffinity column. The folic acid binds to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Folic acid then eluted and injected into an analytical instrument for detection.

2. Components of the kit:

Each kit contains folic acid immunoaffinity columns and 1 instruction manual.

3. Necessary items not provided in the box:

Equipment

HPLC /LC-MS（LC-MS/MS）
Nitrogen gas evaporatorapparatus
Nitrogen gas tank and pressureregulator
Air-pressure controller bracket
Air pump
Balance with 0.01greadability
Ultrasoniccleaner
High-speed freezing centrifuge (can be set to 10℃, 6000RPM)
Centrifugetube:50-mL
Constant temperature incubator or water bath (can be set to 35–37℃)
High-speed homogenizer (maximum speed> 10,000 RPM) or shaker
Grinder
pH meter (or pH testpaper)
Volumetric flask: 100 mL/50mL/250mL/500mL/1000mL
Syringe: 10 mL/20mL
Homogenization flask (or 250-mL conical flask withpestle)
Vials andtubes
Pipette: 1 mL /10 mL and pipettetips
Column holder and syringe connector plug (for use with immunoaffinitycolumns)

3.2 Reagents

Methanol (CH₃OH): Chromatographygrade
Sodium hydroxide (NaOH): AnalyticalGrade
Sodium chloride (NaCl): AnalyticalGrade
Hydrochloric acid (HCl): ChromatographyGrade
Potassium dihydrogen phosphate (KH₂PO₄): AnalyticalGrade
Dipotassium phosphate (K₂HPO₄): AnalyticalGrade
Sodium carbonate (Na₂CO₃): AnalyticalGrade
Sodium bicarbonate (NaHCO₃): AnalyticalGrade
Pancreatin:≥1U/mg
L-ascorbic acid sodium salt(C₆H₇O₆Na): AnalyticalGrade
Distilled/deionizedwater

4. Note:

Allow the immunoaffinity column to return to room temperature (22 to 25°C) before
The affinity column should be stored at 2 to 8°C, do not
Dot use any expired immunoaffinity
Column capacity:500 ng, when the content of the folic acid in the sample divided by the dilution factor is higher than the column capacity, it is necessary to reduce the volume of the sample solution appropriately, and
The pH of the loading solution of the affinity column should be between 6 to 8. If it deviates from this range, the pH should be adjusted with dilute hydrochloric acid or dilute sodium

Keeping the solvent and mobile phase consistent with the instrument detection can eliminate the effect of solvent effects.