Aflatoxins are toxic metabolites of a class of fungi (such as Aspergillus flavus and Aspergillus parasiticus). They are highly carcinogenic. Zearalenone (ZEN), also known as F-2 toxin, mainly pollutes crops such as corn, wheat, and grains. It has a strong estrogenic effect and can cause hyperestrogen, as well as severe reproductive tract symptoms and infertility, and is also immunotoxic and genotoxic. Deoxynivalenol (DON) also known as Vomitoxin, belongs to the thichothecene family. Due to its cytotoxicity and immunosuppressive properties, it poses a health threat to both humans and animals. Ochratoxin A is produced by a variety of Aspergillus and Penicillium growing on crops such as food, peanuts, beans, etc. Ochratoxins mainly have detrimental effects on the liver and kidneys of animals, hence causing liver damage, and have teratogenic effects.
The basis of the IAC measurement is the antigen-antibody interaction. All four main toxin antibodies are embedded throughout the column. After a sample is extracted and filtered, it is slowly passed through the IAC. The toxins bind to their corresponding antibody in the column. The IAC is then washed to remove unbound substances. The toxins are then eluted with the eluent, concentrated using nitrogen gas, then injected into an analytical instrument for detection. Affinity columns can be used in combination with HPLC and LC-MSMS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.
This kit is manufactured to the international quality standard ISO 9001.
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Combination of Immunoaffinity Column with high performance liquid chromatography (HPLC) or LC-MS/MS is a quantitative method for the detection of mycotoxins in samples such as grains, nuts, feed, dairy and more. Samples are purified by the column and then analyzed with HPLC or LC-MSMS.