Background:
Sulfonamides are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.
HPLC/GC-MS method is now widely used in the detection of sulfonamides residues, but its high cost and complex operation make it unacceptable.
Use Principles:
Sulfonamides ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The sulfonamides antigen is precoated on the wells. The precoated antigen compete the sulfonamides antibody (antibody solution) with sulfonamides in the sample, anti- sulfonamides antibody binds to the sulfonamides-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the sulfonamides concentration of the samples is derived.
| Manufacturer | Meizheng |
| Order No. | ME08B |
| Analyte | Sulfonamides |
| Sample Type | Tissue, aquatic tissue, serum, etc. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Sulfadiazine 100% Sulfadoxine 181% Sulfamonomethoxine 150% Phthalylsulfathiazole (PST) 90% Sulfamethoxydiazine 85% Sulfadimethoxypyrimidine 67% Benzene Sulfapyridine 62% Sulfapyridine 55% Sulfamerazine 52% Sulfamethizole 49% Sulfachloropyridazine 29.6% Sulfamethazine 13% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 0.5 ppb |
| Limit of detection | Tissue samples 2ppb Serum sample 5ppb |
| Percent Recovery, % | Tissue samples………………………80±10% Serum sample………………………90±15% |
| Precision (CV %): | Intra-lab assay: CV%≤10% |
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 96 T |
| 5 vials of standard | 1mL each |
| High concentration standard 1ppm | 1mL |
| SAs Enzyme Conjugate | 6mL |
| SAs Antibody Solution | 9mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| SAs Concentrated Sample Diluting Buffer I (10×) | 45mL |
| SAs Concentrated Sample Diluting Buffer II (2×) | 45mL |
| IFU | 1 |
| Antigen coated plate | 96 T |
| 5 vials of standard | 1mL each |
Note:The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb and 13.5ppb.
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Oscillator
—- Vortex
—- Rotary evaporator/nitrogen evaporator
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Graduated pipette: 10mL
—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30µL-300µL)
- Reagent:
—- Methanol (AR)
—- N-hexane (AR)
—- Deionized water
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
