Summary
Sulfonamides are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.
HPLC/GC-MS method is now widely used in the detection of sulfonamides residues, but its high cost and complex operation make it unacceptable.
Order No.: ME08EER
Sensitivity: 0.5 ppb
Package: 96T
Use Principles
Sulfonamides ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The sulfonamides antigen is precoated on the wells. The precoated antigen compete the sulfonamides antibody (antibody solution) with sulfonamides in the sample, anti- sulfonamides antibody binds to the sulfonamides-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the sulfonamides concentration of the samples is derived.
Cross-reactivity
Sulfadiazine 100%
Sulfadoxine 181%
Sulfamonomethoxine 150%
Phthalylsulfathiazole (PST) 90%
Sulfamethoxydiazine 85%
Sulfadimethoxypyrimidine 67%
Benzene Sulfapyridine 62%
Sulfapyridine 55%
Sulfamerazine 52%
Sulfamethizole 49%
Sulfachloropyridazine 29.6%
Sulfamethazine 13%
Materials Required But Not Provided
1.Devices:
—- Microwell plate Reader (450nm/630nm)
—- Oscillator
—- Vortex
—- Rotary evaporator/nitrogen evaporator
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Graduated pipette: 10mL
—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30µL-300µL)
- Reagent:
—- Methanol (AR)
—- N-hexane (AR)
—- Deionized water
—- Concentrated sulfuric acid (18 mol/L)
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 96 T |
| 5 vials of standard | 1mL each |
| High concentration standard 1ppm | 1mL |
| SAs Enzyme Conjugate | 6mL |
| SAs Antibody Solution | 9mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| SAs Concentrated Sample Diluting Buffer I (10×) | 45mL |
| SAs Concentrated Sample Diluting Buffer II (2×) | 45mL |
| IFU | 1 |
Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb and 13.5ppb.
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 6 vials of standard | 1mL each |
| High concentrated standard (100ppb) | 1mL |
| AHD Enzyme Conjugate | 6mL |
| AHD Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Concentrated sample diluting buffer (2X) | 40mL |
| Concentrated derivatization reagent10X) | 1mL |
| IFU | 1 |
| Testing Report | 1 |
| Plate cover sheet | 2 |
Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
