Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.
Furazolidone Metabolite (AOZ) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The AOZ antigen is precoated on the wells. The precoated antigen compete the AOZ antibody (antibody solution) with AOZ in the sample, anti-AOZ antibody binds to the AOZ-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the AOZ concentration of the samples is derived.
|Sample Type||Honey, milk, etc.|
|Methodology||Indirect competitive enzyme-labeled immunoassay.|
|Storage||The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!|
|Shelf life||The validity for this kit is 12 months|
|Lead time||Shipped within 5-12 working days.|
|Cross Reactivity||Furazolidone metabolite (AOZ) 100%|
Furantoin metabolite (AHD)<0.1%
Furaltadone metabolite (AMOZ)<0.1%
Furacilin metabolite (SEM)<0.1%
|Calculate Result||Analyze the result by using the software|
|Limit of detection||0.1ppb|
|Percent Recovery, %||100±20%|
|Precision (CV %):||Intra-lab assay: CV<10%|
Provided Materials and Reagent
|Antigen coated plate||96 wells|
|6 vials of standard||1mL each|
|High concentration standard (100ppb)||1mL|
|Concentrated wash buffer (10×)||40mL|
|Concentrated sample diluting buffer (2×)||40mL|
|Concentrated derivatization reagent (10×)||1mL each|
|Plate cover sheet||2|
Note: The concentrations of the standards are 0ppb, 0.03ppb, 0.09ppb, 0.27ppb, 0.81ppb and 2.43ppb.
Materials Required But Not Provided
---- Microwell plate Reader (450nm/630nm)
---- Vortex mixer
---- Balance (sensibility: 0.01g)
---- Nitrogen evaporator
---- Water bath
---- Rubber bulb
---- Transfer pipette: 10mL
---- Centrifuge tube: 7mL/10mL, 50mL
---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL) Multi-channel pipette (30-300µL)
---- Ethyl acetate (AR)
---- Deionized/distilled water
---- N-hexane (AR)
---- Methanol (AR)
---- Concentrated hydrochloric acid (AR)
---- NaOH (AR)
---- Dipotassium Hydrogen Phosphate Trihydrate (K2HPO4.3H2O) (AR)
---- Concentrated sulfuric acid (18mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.