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Milk and Honey Furazolidone Metabolite ELISA Test Kit

Qualitative or quantitative analysis of furazolidone metabolite in honey, milk, etc.

Brochure-Dairy Test. PDF

Brochure-Honey Test. PDF

Meizheng Bio-Tech Company Overview. PDF


Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

Use Principles:

Furazolidone Metabolite (AOZ) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The AOZ antigen is precoated on the wells. The precoated antigen compete the AOZ antibody (antibody solution) with AOZ in the sample, anti-AOZ antibody binds to the AOZ-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the AOZ concentration of the samples is derived.

Order No.SC0043
Analyte Furazolidone Metabolite
Sample TypeHoney, milk, etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityFurazolidone metabolite (AOZ) 100%
Furantoin metabolite (AHD)<0.1%
Furaltadone metabolite (AMOZ)<0.1%
Furacilin metabolite (SEM)<0.1%
 Calculate ResultAnalyze the result by using the software
Sensitivity   0.03ppb
Limit of detection0.1ppb
Percent Recovery, %100±20%
Precision (CV %):Intra-lab assay: CV<10%

Provided Materials and Reagent

Antigen coated plate96 wells
6 vials of standard1mL each
High concentration standard (100ppb)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated wash buffer (10×)40mL
Concentrated sample diluting buffer (2×)40mL
Concentrated derivatization reagent (10×)1mL each
Testing Report1
Plate cover sheet2

Note: The concentrations of the standards are 0ppb, 0.03ppb, 0.09ppb, 0.27ppb, 0.81ppb and 2.43ppb.

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex mixer

—- Centrifuge

—- Incubator

—- Balance (sensibility: 0.01g)

—- Nitrogen evaporator

—- Water bath

—- Rubber bulb

—- Transfer pipette: 10mL

—- Centrifuge tube: 7mL/10mL, 50mL

—- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL) Multi-channel pipette (30-300µL)

  1. Reagent:

—- Ethyl acetate (AR)

—- Deionized/distilled water

—- N-hexane (AR)

—- Methanol (AR)

—- Concentrated hydrochloric acid (AR)

—- NaOH (AR)

—- Dipotassium Hydrogen Phosphate Trihydrate (K2HPO4.3H2O) (AR)

—- Concentrated sulfuric acid (18mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.


1-10, 11-100, 101-1000, 1001-10000


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