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    Pesticides Sodium Pentachlorophenol ELISA Test Kit for Meat

    【Summary】

    Sodium Pentachlorophenol is the sodium salt of pentachlorophenol, which belongs to organochlorine pesticides and can be used as insecticides, antibacterial agents, and preservatives. Sodium pentachlorophenate is easy to accumulate in animals and can enter the human body through the food chain, seriously endangering human health.

    Use Principles

    Sodium Pentachlorophenol ELISA Kit is an indirect competitive

    enzyme-labeled immunoassay. The Sodium Pentachlorophenol antigen is precoated on the wells. The precoated antigen compete the Sodium Pentachlorophenol antibody (Antibody Solution) with Sodium Pentachlorophenol in the sample, Sodium Pentachlorophenol antibody binds to the Sodium Pentachlorophenol Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Sodium Pentachlorophenol contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Sodium Pentachlorophenol in the sample can be calculated.

    Scope of Application

    Qualitative or quantitative analysis of Sodium Pentachlorophenol concentrations in samples of chicken, and duck.

    【Cross Reactivity】

    Sodium Pentachlorophenol 100%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Vortex

    ---- Termovap Sample Concentrator

    ---- Oscillator

    ---- Centrifuge

    ---- Constant Temperature Incubator

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Rubber Bulb Syringe

    ---- Centrifuge tube: 10mL,50mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL).  Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- Sodium Hydroxide (NaOH) (AR)

    ---- Ethyl Acetate (AR)

    ---- 18M Concentrated Sulfuric Acid (H2SO4)

    【Provided Materials and Reagent】

    Component96 wells
    Antigen coated plate96T
    5 vials of Standard2mL each
    High Concentration Standard (1ppm)2mL
    Enzyme Conjugate12mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer (8×)40mL
    Package Insert1
    Testing Report1
    Plate cover sheet2

    Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, and 5.4ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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