【Summary】
Sodium Pentachlorophenol is the sodium salt of pentachlorophenol, which belongs to organochlorine pesticides and can be used as insecticides, antibacterial agents, and preservatives. Sodium pentachlorophenate is easy to accumulate in animals and can enter the human body through the food chain, seriously endangering human health.
【Use Principles】
Sodium Pentachlorophenol ELISA Kit is an indirect competitive
enzyme-labeled immunoassay. The Sodium Pentachlorophenol antigen is precoated on the wells. The precoated antigen compete the Sodium Pentachlorophenol antibody (Antibody Solution) with Sodium Pentachlorophenol in the sample, Sodium Pentachlorophenol antibody binds to the Sodium Pentachlorophenol Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Sodium Pentachlorophenol contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Sodium Pentachlorophenol in the sample can be calculated.
【Scope of Application】
Qualitative or quantitative analysis of Sodium Pentachlorophenol concentrations in samples of chicken, and duck.
【Cross Reactivity】
Sodium Pentachlorophenol 100%
【Materials Required But Not Provided】
- Devices:
—- Microplate Reader (450nm/630nm filter)
—- Vortex
—- Termovap Sample Concentrator
—- Oscillator
—- Centrifuge
—- Constant Temperature Incubator
—- Balance (sensibility: 0.01g)
—- Graduated pipette: 10mL
—- Rubber Bulb Syringe
—- Centrifuge tube: 10mL,50mL
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL). Multi-channel pipette (30-300µL)
- Reagent:
—- Deionized water
—- Sodium Hydroxide (NaOH) (AR)
—- Ethyl Acetate (AR)
—- 18M Concentrated Sulfuric Acid (H2SO4)
【Provided Materials and Reagent】
Component | 96 wells |
Antigen coated plate | 96T |
5 vials of Standard | 2mL each |
High Concentration Standard (1ppm) | 2mL |
Enzyme Conjugate | 12mL |
Antibody Solution | 6mL |
Substrate A | 6mL |
Substrate B | 6mL |
Concentrated Wash Buffer (10×) | 40mL |
Concentrated Sample Diluting Buffer (8×) | 40mL |
Package Insert | 1 |
Testing Report | 1 |
Plate cover sheet | 2 |
Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, and 5.4ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.