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    Qualitative or Quantitative Salbutamol ELISA Test Kit

    Qualitative or quantitative analysis of Salbutamol in swine urine, animal tissue, swine serum, feed, and other samples.

    Order No.: ME31AER

    【Summary】

    Salbutamol, also known as albuterol, it is a β-agonist drug, which belongs to a group of synthetic compounds affecting β-adrenergic agonist receptors located in all body tissues, including the skeletal muscle. The positive effects of β-agonist as a feed additive in farm animals, including improved feed efficiency, increased growth rate, altered adipose accretion, increased muscle mass in meat producing animals. In the early nineties, following intoxication outbreaks attributed to consumption of livers from cattle treated with β-agonist, salbutamol is forbidden to use in food animals by the European Union.

    【Principles】

    Salbutamol ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Salbutamol antigen is precoated on the wells. The precoated antigen compete the Salbutamol antibody (Antibody Solution) with Salbutamol in the sample, Salbutamol antibody binds to the Salbutamol Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Salbutamol contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Salbutamol in the sample can be calculated.

    【Cross Reactivity】

    Salbutamol 100%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Constant Temperature Incubator (Optional)

    ---- Homogenizer

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Graduated Pipette: 10mL

    ---- Rubber Bulb Syringe

    ---- Centrifuge tube: 5mL/7mL, 50mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized Water

    ---- Methanol (AR)

    ---- Trichloroacetic Acid (CCl3COOH) (AR)

    ---- 18M Concentrated Sulfuric Acid (H2SO4)

    【Provided Materials and Reagent】

    Component 96 wells
    Antigen Coated Plate96T
    6 vials of Standard1mL each
    High Concentration Standard (100ppb)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer Ⅰ50mL
    Concentrated Sample Diluting Buffer Ⅱ15mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.1ppb, 0.3ppb, 0.9ppb, 2.7ppb, 8.1ppb.

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