Salbutamol ELISA Test Kit for Swine Urine, Animal Tissue, Swine Serum and Feed

Summary

Salbutamol, also known as albuterol, it is a β-agonist drug, which belongs to a group of synthetic compounds affecting β-adrenergic agonist receptors

located in all body tissues, including the skeletal muscle. The positive effects of β-agonist as a feed additive in farm animals, including improved feed

efficiency, increased growth rate, altered adipose accretion, increased muscle mass in meat producing animals. In the early nineties, following

intoxication outbreaks attributed to consumption of livers from cattle treated with β-agonist, salbutamol is forbidden to use in food animals by the

European Union.

Use Principles

Salbutamol ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Salbutamol antigen is precoated on the wells. The precoated

antigen compete the Salbutamol antibody (Antibody Solution) with Salbutamol in the sample, Salbutamol antibody binds to the Salbutamol Enzyme

Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Salbutamol contained

in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Salbutamol in the sample can be

calculated.

SENSITIVITY (DETECTION LIMIT)

—- Sensitivity: 0.1ppb

Limit of detection

Swine urine, animal tissue, swine serum …………………………………………0.5ppb

Feed ………………………………………………………………………………………………..5ppb

Cross-reactivity

Salbutamol …………………………………………………………………………………….. 100%

Percent Recovery, %: 100±20%

recision (CV %): Intra-lab assay: CV%≤10%

Kit Contents

Kit Contents	Quantity	Storage
Antigen Coated Plate	96T	2-8°C
6 vials of Standard	1mL each	2-8°C
High Concentration Standard (100ppb)	1mL	2-8°C
Enzyme Conjugate	6mL	2-8°C
Antibody Solution	6mL	2-8°C
Substrate A Substrate B	6mL 6mL	2-8°C 2-8°C
Concentrated Wash Buffer (10× )	40mL	2-8°C
Concentrated Sample Diluting Buffer Ⅰ	50mL	—
Concentrated Sample Diluting Buffer Ⅱ	15mL	—
Package Insert	1	—
Testing Report	1	—
Plate Cover Sheet	2	—