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Seafood and Water Illegal Additive Malachite Green ELISA Test Kit

Qualitative or quantitative analysis of malachite green, leuco-malachite green, crystal violet and leuco-crystal violet residues in seafood (fish, shrimp), water, etc.

Order No.: ME02ER

Summary 

Residues of malachite green (MG), a veterinary drug illegally used for the treatment of aqua-cultured fish. The detection of illegal additive residues such as malachite green in seafood and water is of great concern for the aquaculture because of the potential interfering and adverse effects of illegal additive residues frequently found in seafood and water.

Use Principles 

Malachite green ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The malachite green antigen is precoated on the wells. The precoated antigen compete the malachite green antibody (antibody solution) with malachite green in the sample, anti- malachite green antibody binds to the malachite green-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the malachite green concentration of the samples is derived.

Cross-reactivity

Malachite green…………………………..………………………100%

Leuco-malachite green (oxidized)………………….………………100%

Crystal violet…………….………………………………………150%

Leuco-crystal violet………………………………………………………………….150%

Materials Required But Not Provided

  1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Homogenizer

—- Vortex

—- Centrifuge

—- Constant Temperature Incubator

—- Balance (sensibility: 0.01g)

—- Graduated pipette: 10mL

—- Termovap Sample Concentrator

—- Rubber Bulb Syringe

—- Centrifuge tube: 5mL/7mL, 50mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

  1. Reagent:

—- Deionized water

—- Acetonitrile (AR)

—- 18M Concentrated Sulfuric Acid (H2SO4)

—- Sodium hydroxide (NaOH) (AR)

Provided Materials and Reagent

Component 96 wells
Antigen coated plate96T
5 vials of standard1mL each
High concentration standard (100ppb)1mL
Antibody Solution6mL
Enzyme Conjugate12mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Sample diluting buffer ×2 vials45mL/vial
Sample extractant A ×2 bags90g/bag
Sample extractant B ×2 bags90g/bag
Oxidizer6mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note:

  1. The concentrations of the standards are 0ppb, 0.03ppb, 0.09ppb, 0.27ppb and 0.81ppb.
  2. Sample extractant A and B: After each test, please seal the zip lock bag and put it in the aluminum foil bag with desiccant for sealing again.

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