Sulfamethazine ELISA Test Kit for Dairy, Meat, Serum, Urine and Feed

Procedure Overview

The method is based on a competitive, two-step colorimetric enzyme-linked immunosorbent assay (ELISA). The target analyte has been coated to the plate wells. During analysis, sample is added along with a primary antibody specific to the target analyte. If the target analyte is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the analyte coated to the plate well. A secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the analyte coated on the plate wells. The resulting color intensity, after addition of TMB substrate, has an inverse relationship to the target analyte concentration in the sample.

Order No: ME08DER

KIT CONTENTS, STORAGE, & SHELF LIFE

ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the entire kit at 2–8°C. Do not use this product past the expiration date indicated on the Certificate of Analysis.

Kit Contents	Quantity	Storage
Sulfamethazine-Coated Plate	1 x 96-well plate	2–8°C
Sulfamethazine Standards
Negative control 0.8 mL		2–8°C
0.25 ng/mL 0.8 mL		2–8°C
0.5 ng/mL 0.8 mL		2–8°C
1.0 ng/mL 0.8 mL		2–8°C
5.0 ng/mL 0.8 mL		2–8°C
10 ng/mL 0.8 mL		2–8°C
Spiking Solution
100 ng/mL (red cap tube)	0.8 mL	2–8°C
Sulfamethazine Antibody #1	12 mL	2–8°C
100X HRP-Conjugated Antibody #2	250 µL	2–8°C
20X Wash Solution	28 mL	2–8°C
Antibody #2 Diluent	20 mL	2–8°C
TMB Substrate	12 mL	2–8°C
10X Sample Extraction Buffer	25 mL	2–8°C

Required Materials Not Provided with the Kit

Microtiter Plate Reader with 450 nm primary filter and optional 630 nm differentialfilter
TissueHomogenizer
VortexMixer
10, 20, 100 and 1000 µLPipettes
Distilled or deionizedwater
Multi-Channel Pipette: 50–300 µL(Optional)
Reagent Resevoir(Optional)
Incubator capable of at least75°C
Centrifuge capable of 4,000 xg
Carrez I: 0.36 M Potassium Hexacyanoferrate (II)Trihydrate
Carrez II: 12M concentrated hydrochloric acid(HCl)