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    Sulfamethazine ELISA Test Kit for Dairy, Meat, Serum, Urine and Feed

    Procedure Overview

    The method is based on a competitive, two-step colorimetric enzyme-linked immunosorbent assay (ELISA). The target analyte has been coated to the plate wells. During analysis, sample is added along with a primary antibody specific to the target analyte. If the target analyte is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the analyte coated to the plate well. A secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the analyte coated on the plate wells. The resulting color intensity, after addition of TMB substrate, has an inverse relationship to the target analyte concentration in the sample.

    Order No: ME08DER

    KIT CONTENTS, STORAGE, & SHELF LIFE

    ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the entire kit at 2–8°C. Do not use this product past the expiration date indicated on the Certificate of Analysis.

    Kit ContentsQuantityStorage
    Sulfamethazine-Coated Plate1 x 96-well plate2–8°C
    Sulfamethazine Standards 
    Negative control 0.8 mL2–8°C
    0.25 ng/mL 0.8 mL2–8°C
    0.5 ng/mL 0.8 mL2–8°C
    1.0 ng/mL 0.8 mL2–8°C
    5.0 ng/mL 0.8 mL2–8°C
    10 ng/mL 0.8 mL2–8°C
    Spiking Solution 
    100 ng/mL (red cap tube)0.8 mL2–8°C
    Sulfamethazine Antibody #112 mL2–8°C
    100X HRP-Conjugated Antibody #2250 µL2–8°C
    20X Wash Solution28 mL2–8°C
    Antibody #2 Diluent20 mL2–8°C
    TMB Substrate12 mL2–8°C
    10X Sample Extraction Buffer25 mL2–8°C

    Required Materials Not Provided with the Kit

    • Microtiter Plate Reader with 450 nm primary filter and optional 630 nm differentialfilter
    • TissueHomogenizer
    • VortexMixer
    • 10, 20, 100 and 1000 µLPipettes
    • Distilled or deionizedwater
    • Multi-Channel Pipette: 50–300 µL(Optional)
    • Reagent Resevoir(Optional)
    • Incubator capable of at least75°C
    • Centrifuge capable of 4,000 xg
    • Carrez I: 0.36 M Potassium Hexacyanoferrate (II)Trihydrate
    • Carrez II: 12M concentrated hydrochloric acid(HCl)

    SENSITIVITY

    Sample TypeDetection Limit (ng/g or ppb)
    Beef1
    Pork1
    Meat, Liver, Kidney2
    Feed1
    Milk10
    Milk Powder11.5
    Serum/Plasma2.5
    Urine1.25

    SPECIFICITY

    AnalyteCross-Reactivity (%)
    Sulfamethazine100

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