Sulfamethazine ELISA Test Kit

Procedure Overview The method is based on a competitive, two-step colorimetric enzyme-linked immunosorbent assay (ELISA). The target analyte has been coated to the plate wells. During analysis, sample is added along with a primary antibody specific to the target analyte. If the target analyte is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the analyte coated to the plate well. A secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the analyte coated on the plate wells. The resulting color intensity, after addition of TMB substrate, has an inverse relationship to the target analyte concentration in the sample.Order No: ME08DER