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    Sulfonamides ELISA Test Kit for Poultry Tissues, Aquatic Tissue and Serum

    Scope of Application

    The Sulfonamides ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of sulfonamides in samples of poultry tissues, aquatic tissue, serum, etc.

    Order No: ME08BER

    Summary

    Sulfonamides are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human.

    They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their

    effectiveness against gram-negative and gram-positive organisms.

    The detection method is sensitive, fast, simple, and specific, only need 45mins for operating and a small amount of sampling.

    Use Principles

    The Sulfonamides ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen

    compete the antibody (antibody solution) with sulfonamides in the sample, anti-sulfonamides antibody binds to the HRP enzyme conjugate. Then

    pipette the substrate solution to the wells to convert the color. The color of unknown samples is compared to the color of the standards and the

    sulfonamides concentration of the samples is derived.

    SENSITIVITY (DETECTION LIMIT)

    ---- Sensitivity: 0.5ppb

    Limit of detection

    Tissue sample ............................................................................................ 4ppb

    Serum sample............................................................................................ 5ppb

    Cross-reactivity

    Sulfadiazine ...............................................................................................100%

    Sulfadoxine ................................................................................................181%

    Sulfamonomethoxine................................................................................150%

    Phthalylsulfathiazole (PST).........................................................................90%

    Sulfamethoxydiazine...................................................................................85%

    Sulfadimethoxypyrimidine .........................................................................67%

    Benzene Sulfapyridine ................................................................................62%

    Sulfapyridine................................................................................................55%

    Sulfamerazine..............................................................................................52%

    Sulfamethizole.............................................................................................49%

    Sulfachlorpyridazine ................................................................................29.6%

    Sulfamethazine............................................................................................13%

    Percent Recovery, %

    Tissue sample ...................................................................................... 80±10%

    Serum sample...................................................................................... 90±15%

    Precision (CV %):

    Intra-lab assay: CV%≤10%

    Kit ContentsQuantityStorage
    Antigen coated plate96T2-8°C
    5 vials of Standard1mL each2-8°C
    High concentration standard (1ppm)1mL2-8°C
    Enzyme Conjugate6mL2-8°C
    Antibody Solution9mL2-8°C
    Substrate A6mL2-8°C
    Substrate B6mL2-8°C
    Concentrated Sample Diluting Buffer I (10× )45mL2-8°C
    Concentrated Sample Diluting Buffer II (2× )45mL
    Concentrated Wash Buffer (10× )40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

     

     

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