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Trimethoprim ELISA Test Kit for Animal Tissues, Aquatic Tissue and Eggs

Qualitative or quantitative analysis of trimethoprim (TMP) residues in animal tissues, aquatic tissue and eggs.

Meizheng Bio-Tech Company Overview. PDF

Background:

Trimethoprim (TMP) is a common sulfonamide synergist because of its weak antibacterial activity. In combination with sulfonamides, the antibacterial activity can be enhanced several times, even dozons of times. TMP can treat infection caused by escherichia coli induced septicemia, typhoid fever, cholera, secondary respiratory infection.

Use Principles: 

Trimethoprim (TMP) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Trimethoprim (TMP) antigen is precoated on the wells. The precoated antigen compete the Trimethoprim (TMP) antibody (antibody solution) with Trimethoprim (TMP)s in the sample, anti- Trimethoprim (TMP) antibody binds to the Trimethoprim-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Trimethoprim (TMP) concentration of the samples is derived.

ManufacturerMeizheng
Order No.ME19A
AnalyteTrimethoprim
Sample TypeAnimal tissues, aquatic tissue and eggs.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityTrimethoprim (TMP)………………………100%
 Calculate ResultAnalyze the result by using the software
Sensitivity 0.5ppb
Limit of detection2ppb
Percent Recovery, %100±30%
Precision (CV %):Intra-lab assay: CV%<10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 test strips of 8 wells each
6 vials of standard1.5mL each
High concentration standard (1ppm)1mL
Enzyme Conjugate12mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Auxiliary Extracting Agent (10×)15mL
Concentrated Sample Diluting Buffer40mL
IFU1
Testing Report1
Plate cover sheet2

 Note:The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb and 40.5ppb.

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Homogenizer

—- Oscillator

—- Vortex

—- Rotary evaporator/nitrogen evaporator

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Centrifuge tube: 5mL/7mL, 50mL

—- Transfer pipette: 10mL

—- Aurilave

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL -300µL)

  1. Reagent:

—- Deionized water

—- Ethyl acetate (AR)

—- N-hexane  (AR)

—- Concentrated sulfuric acid (18 mol/L)

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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