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Veterinary Drug Residues Sulfamethazine ELISA Test Kit

Product Description

Sulfamethazine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of sulfamethazine. Sulfonamides are widely used as feed additives, mainly for fattening of calves and pigs. Sulfonamides are also used in veterinary medicine for the treatment of intestinal infections, mastitis, and other diseases. Sulfonamide residues may therefore occur in food of animal origin such as fish and shrimp. According to the US FDA and EU-Law, a maximum residue limit for all substances of the sulfonamide-group of 100 ppb in muscle, fat, liver, kidney, and milk.

Although physical-chemical methods such as high-pressure liquid chromatography are widely used for sulfamethazine detection, their application for routine screening of numerous samples, however, is limited by cost and instrumentation. Alternatively, enzyme immunoassays (ELISA) show considerable advantages over conventional methods. Sulfamethazine ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect

Sulfamethoxazole below the maximum residue limit in various sample types in response to customer concerns about food safety.

Procedure Overview

The method is based on a competitive, two-step colorimetric enzyme-linked immunosorbent assay (ELISA). The target analyte has been coated to the plate wells. During analysis, sample is added along with a primary antibody specific to the target analyte. If the target analyte is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the analyte coated to the plate well. A secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the analyte coated on the plate wells. The resulting color intensity, after addition of TMB substrate, has an inverse relationship to the target analyte concentration in the sample




Sulfamethazine ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the entire kit at 2–8°C. Do not use this product past the expiration date indicated on the Certificate of Analysis.

Kit ContentsQuantityStorage
Sulfamethazine-Coated Plate1× 96-well plate2–8°C
Sulfamethazine Standards  
Negative control (white cap tube)0.8 mL2–8°C
0.25 ng/mL (yellow cap tube)0.8 mL2–8°C
0.5 ng/mL (orange cap tube)0.8 mL2–8°C
1.0 ng/mL (pink cap tube)0.8 mL2–8°C
5.0 ng/mL (purple cap tube)0.8 mL2–8°C
10 ng/mL (blue cap tube)0.8 mL2–8°C
Spiking Solution  
100 ng/mL (red cap tube)0.8 mL2–8°C
Sulfamethazine Antibody #112 mL2–8°C
100× HRP-Conjugated Antibody #2250 µL2–8°C
20× Wash Solution28 mL2–8°C
Antibody #2 Diluent20 mL2–8°C
TMB Substrate12 mL2–8°C
10× Sample Extraction Buffer25 mL2–8°C

Required Materials Not Provided with the Kit

  •  Microtiter Plate Reader with 450 nm primary filter and optional 630 nm differential filter
  •  Tissue Homogenizer
  •  Vortex Mixer
  •  10, 20, 100 and 1000 µL Pipettes
  •  Distilled or deionized water
  •  Multi-Channel Pipette: 50–300 µL (Optional)
  •  Reagent Resevoir (Optional)
  •  Incubator capable of at least 75°C
  •  Centrifuge capable of 4,000 × g
  •  Carrez I: 0.36 M Potassium Hexacyanoferrate (II) Trihydrate
  •  Carrez II: 1.04 M Zinc Sulfate Heptahydrate
  •  12M concentrated hydrochloric acid (HCl)


Sample TypeDetection Limit (ng/g or ppb)
Meat, Liver, Kidney2
Milk Powder11.5

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.


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