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Wheat and Flour Mycotoxin Deoxynivalenol (DON) ELISA Test Kit

The Deoxynivalenol ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Deoxynivalenol in rice flour, wheat, barley, corn, flour, oats etc.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF


Deoxynivalenol is known as vomitoxin or DON. It is a potent fungal toxin to affect crops grown in cool and moist conditions. Fusarium graminearum is the principal DON-producing fungus in grains but Fusarium culmorum is often involved as well especially in certain geographical areas of the world. Deoxynivalenol is a known immunosuppressant and may cause kidney problems. Consuming DON-contaminated grain can cause vomiting in humans and animals. Deoxynivalenol is formed by the Fusarium species and mainly affects corn, wheat, oats and barley.

Order No.HEM0896
Sample TypeFlour, Wheat, Barley, Corn, Flour, Oats, etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityDON…………………………………………100%
Oxynivalenol …………………………………<1%
Result InterpretationAnalyze the result by using the software
Sensitivity4 ppb
Limit of detection100ppb
Percent Recovery  110±15%
Intra-lab assayCV%≤10%

 Provided Materials and Reagent:

Component96 wells
Antigen coated plate12 X 8 wells
5 vials of Standard1mL each
DON Enzyme Conjugate6mL
DON Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Stop Solution6mL
Concentrated Wash Solution (10X)40mL
Package Insert1
Plate Cover Sheet2
Testing Report1

Note: The concentrations of the standards are 0µg/kg, 4µg/kg, 20µg/kg, 80µg/kg and 200µg/kg respectively.

Materials Required But Not Provided


—- Microwell Plate Reader (450nm/630nm)

—- Oscillator/ Vortex

—- Centrifuge (or funnel)

—- Balance (sensibility: 0.01g)

—- Pipette: 10mL

—- Centrifuge tube: 50mL (or triangular flask with stopper)

—- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL-300µL)


—- Deionized water

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.


1-10, 11-100, 101-1000, 1001-10000


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