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    Aflatoxins Total ELISA Test Kit for Grains and Feed

    The Aflatoxin Total ELISA Test Kit is a competitive ELISA for the quantitative analysis of aflatoxin in grain, nuts, cottonseed, and feed products.

    Brochure-Grains & Feed Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

    ManufacturerMeizheng
    TrademarkToxinFast®
    Order No.HEM0196
    AnalyteAflatoxin Total
    Sample TypeGrain, nuts, cottonseed and feed
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityAflatoxin B2 ---107%
    Aflatoxin B1 ---100%
    Aflatoxin G1 ---77%
    AflatoxinG2 ---75%
    Result InterpretationAnalyze the result by using the software
    Sensitivity0.035ppb
    Limit of detectionPeanut, feed, edible oil, corn and other grains……………1.05ppb
    Percent Recovery  90±15%
    Intra-lab assayCV%≤10%

    Provided Materials and Reagent:

    Component96 wells
    Antigen coated plate12 x 8 wells
    6 vials of Standard1mL each
    AFT Enzyme Conjugate6mL
    AFT Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10X)40mL
    Package insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standard are 0ppb, 0.035ppb, 0.09ppb, 0.22ppb, 0.55ppb and 1.35ppb.

    Materials Required But Not Provided:

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Aurilave

    ---- Funnel

    ---- Separating funnel

    ---- Centrifuge tube (10mL, 50mL)

    ---- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    Multi-channel pipet (300µL)

    1. Reagents:

    ---- Methanol

    ---- Deionized water

    ---- Concentrated sulfuric acid (18 mol/L)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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