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Aflatoxins Total ELISA Test Kit for Grains and Feed

The Aflatoxin Total ELISA Test Kit is a competitive ELISA for the quantitative analysis of aflatoxin in grain, nuts, cottonseed, and feed products.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

ManufacturerMeizheng
TrademarkToxinFast®
Order No.HEM0196
AnalyteAflatoxin Total
Sample TypeGrain, nuts, cottonseed and feed
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityAflatoxin B2 —107%
Aflatoxin B1 —100%
Aflatoxin G1 —77%
AflatoxinG2 —75%
Result InterpretationAnalyze the result by using the software
Sensitivity0.035ppb
Limit of detectionPeanut, feed, edible oil, corn and other grains……………1.05ppb
Percent Recovery  90±15%
Intra-lab assayCV%≤10%

Provided Materials and Reagent:

Component96 wells
Antigen coated plate12 x 8 wells
6 vials of Standard1mL each
AFT Enzyme Conjugate6mL
AFT Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10X)40mL
Package insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standard are 0ppb, 0.035ppb, 0.09ppb, 0.22ppb, 0.55ppb and 1.35ppb.

Materials Required But Not Provided:

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Aurilave

—- Funnel

—- Separating funnel

—- Centrifuge tube (10mL, 50mL)

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (300µL)

  1. Reagents:

—- Methanol

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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