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    Animal Tissue Antibiotic Ofloxacin ELISA Test Kit

    Qualitative or quantitative analysis of Ofloxacin in animal tissue (pork,chicken, duck, fish, shrimp, chicken heart, chicken liver, chicken, gizzard).

    Order No.: ME11CER

    LOD: 0.06 ppb

    Package: 96T

    [Summary]

    Ofloxacin (OFL) is a synthetic third-generation quinolone antibacterial

    drug with broad-spectrum antibacterial activity and good bactericidal

    effect. It has antibacterial effect on Enterobacter, Pseudomonas

    aeruginosa, Haemophilus influenzae, Neisseria gonorrhoeae,

    Streptococcus, Legionella, and Staphylococcus aureus.

    The Ofloxacin ELISA Test Kit produced by Meizheng is fast,

    easy-to-use, accurate and has high sensitivity, with 45 minutes

    operation time, which can minimize the operating error and working

    intensity.

    [Principles]

    Ofloxacin ELISA Kit is an indirect competitive enzyme-labeled

    immunoassay. The Ofloxacin antigen is precoated on the wells. The

    precoated antigen compete the Ofloxacin antibody (Antibody Solution)

    with Ofloxacin in the sample, Ofloxacin antibody binds to the

    Ofloxacin Enzyme Conjugate. After the color development of TMB

    Substrate, the absorbance value of the sample is negatively correlated

    with the Ofloxacin contained in it. Compared with the standard curve

    and multiplied by its corresponding dilution factor, the content of

    Ofloxacin in the sample can be calculated.

    [Cross Reactivity]

    Ofloxacin (OFL) ······················································100%

    Ciprofloxacin (CIP) ··················································· <1%

    Enrofloxacin (ENR) ·····················································2%

    Norfloxacin (NOR) ····················································<1%

    Salafloxacin (SAR) ····················································<1%

    Pefloxacin (PEF) ························································ 3%

    Lomefloxacin (LOM) ················································· <1%

    Danfloxacin (DAN) ··················································· <1%

    Enoxacin (ENO) ······················································· <1%

    Mapofloxacin (MAR) ················································130%

    Difloxacin (DIF) ······················································· 28%

    Flumequine ·····························································<1%

    Nafloxacin ······························································<1%

    Oxalic Acid ·····························································<1%

    Obifloxacin ·····························································<1%

     [Materials Required But Not Provided]

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Vortex

    ---- Constant Temperature Incubator (Optional)

    ---- Centrifuge

    ---- Homogenizer

    ---- Balance (sensibility: 0.01g)

    ---- Rubber Bulb Syringe

    ---- Termovap Sample Concentrator

    ---- Centrifuge tube: 5mL/7mL, 50mL

    ---- Graduated Pipette: 10mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized Water

    ---- Acetonitrile (AR)

    ---- N-hexane (AR)

    ---- Concentrated Hydrochloric Acid (HCl) (AR)

    ---- 18M Concentrated Sulfuric Acid (H2SO4)

    [Provided Materials and Reagent]

    Component96 wells
    Antigen Coated Plate96T
    5 vials of Standard1mL each
    High Concentration Standard (100ppb)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer15mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.06ppb, 0.18ppb, 0.54ppb,1.62ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

     

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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