Order No.: ME11CER
LOD: 0.06 ppb
Package: 96T
[Summary]
Ofloxacin (OFL) is a synthetic third-generation quinolone antibacterial
drug with broad-spectrum antibacterial activity and good bactericidal
effect. It has antibacterial effect on Enterobacter, Pseudomonas
aeruginosa, Haemophilus influenzae, Neisseria gonorrhoeae,
Streptococcus, Legionella, and Staphylococcus aureus.
The Ofloxacin ELISA Test Kit produced by Meizheng is fast,
easy-to-use, accurate and has high sensitivity, with 45 minutes
operation time, which can minimize the operating error and working
intensity.
[Principles]
Ofloxacin ELISA Kit is an indirect competitive enzyme-labeled
immunoassay. The Ofloxacin antigen is precoated on the wells. The
precoated antigen compete the Ofloxacin antibody (Antibody Solution)
with Ofloxacin in the sample, Ofloxacin antibody binds to the
Ofloxacin Enzyme Conjugate. After the color development of TMB
Substrate, the absorbance value of the sample is negatively correlated
with the Ofloxacin contained in it. Compared with the standard curve
and multiplied by its corresponding dilution factor, the content of
Ofloxacin in the sample can be calculated.
[Cross Reactivity]
Ofloxacin (OFL) ······················································100%
Ciprofloxacin (CIP) ··················································· <1%
Enrofloxacin (ENR) ·····················································2%
Norfloxacin (NOR) ····················································<1%
Salafloxacin (SAR) ····················································<1%
Pefloxacin (PEF) ························································ 3%
Lomefloxacin (LOM) ················································· <1%
Danfloxacin (DAN) ··················································· <1%
Enoxacin (ENO) ······················································· <1%
Mapofloxacin (MAR) ················································130%
Difloxacin (DIF) ······················································· 28%
Flumequine ·····························································<1%
Nafloxacin ······························································<1%
Oxalic Acid ·····························································<1%
Obifloxacin ·····························································<1%
[Materials Required But Not Provided]
- Devices:
—- Microplate Reader (450nm/630nm filter)
—- Vortex
—- Constant Temperature Incubator (Optional)
—- Centrifuge
—- Homogenizer
—- Balance (sensibility: 0.01g)
—- Rubber Bulb Syringe
—- Termovap Sample Concentrator
—- Centrifuge tube: 5mL/7mL, 50mL
—- Graduated Pipette: 10mL
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
- Reagent:
—- Deionized Water
—- Acetonitrile (AR)
—- N-hexane (AR)
—- Concentrated Hydrochloric Acid (HCl) (AR)
—- 18M Concentrated Sulfuric Acid (H2SO4)
[Provided Materials and Reagent]
Component | 96 wells |
Antigen Coated Plate | 96T |
5 vials of Standard | 1mL each |
High Concentration Standard (100ppb) | 1mL |
Enzyme Conjugate | 6mL |
Antibody Solution | 6mL |
Substrate A | 6mL |
Substrate B | 6mL |
Concentrated Wash Buffer (10×) | 40mL |
Concentrated Sample Diluting Buffer | 15mL |
Package Insert | 1 |
Testing Report | 1 |
Plate Cover Sheet | 2 |
Note: The concentrations of the standards are 0ppb, 0.06ppb, 0.18ppb, 0.54ppb,1.62ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.