Animal Tissue High Sensitively Furacilin Metabolite ELISA Test Kit

Order No.: ME05DER

LOD: 0.03 ppb

Package: 96T

Summary

Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

Use Principles

Furacilin Metabolite ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Furacilin Metabolite antigen is precoated on the wells. The precoated antigen compete the Furacilin Metabolite antibody (Antibody Solution) with Furacilin Metabolite in the sample, Furacilin Metabolite antibody binds to the Furacilin Metabolite Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Furacilin Metabolite contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Furacilin Metabolite in the sample can be calculated.

Cross-reactivity

Furacilin Metabolite………………………………………100%

Furaltadone Metabolite……………………………………<0.1%

Furantoin Metabolite………………………………………<0.1%

Furazolidone Metabolite…………………………………<0.1%

Materials Required But Not Provided

Devices:

—- Microplate Reader (450nm/630nm filter)

—- Oscillator

—- Vortex

—- Centrifuge

—- Homogenizer

—- Constant Temperature Incubator

—- Water Bath

—- Termovap Sample Concentrator

—- Balance (sensibility: 0.01g)

—- Graduated Pipette: 10mL

—- Rubber Bulb Syringe

—- Centrifuge Tube: 10mL or 7mL, 50mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

Reagent:

—- Deionized Water

—- Ethyl Acetate (AR)

—- N-hexane (AR)

—- Methanol (AR)

—- Concentrated hydrochloric acid (HCl) (AR)

—–Sodium hydroxide (NaOH) (AR)

—- Dipotassium hydrogen phosphate trihydrate (K2HPO4·3H2O) (AR)

—- 18M concentrated sulfuric acid (H2SO4)

[Provided Materials and Reagent]

Note: The concentrations of the standards are 0ppb, 0.03ppb, 0.09ppb, 0.27ppb, 0.81ppb,2.43ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.