Background:
The quinolones are a family of synthetic broad-spectrum antibiotics. The majority of quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.
Use Principles:
Quinolones ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The quinolones antigen is precoated on the wells. The precoated antigen compete the quinolones antibody (antibody solution) with quinolones in the sample, anti- quinolones antibody binds to the quinolones-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.
| Manufacturer | Meizheng |
| Order No. | ME07B |
| Analyte | Quinolones |
| Sample Type | Animal tissues, eggs, etc. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Ciprofloxacin (CIF) 25% Enrofloxacin (ENR) 100% Nadifloxacin (NAD) 323% Enoxacin (ENO) 37% Lomefloxacin (LOM) 21% Norfloxacin (NOR) 258% Marbolxacin (MAR) 19% Pefloxacin (PEF) 135% Ofloxacin (OFL) 55% Danofloxain (DAN) 69% Orbifloxacin(ORB) 17% Sarafloxacin (SAR) 33% Oxolinic acid (OXO) 51% Flumequine (FLU) 143% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 0.2 ppb |
| Limit of detection | Tissue, egg 0.4ppb |
| Percent Recovery, % | Tissues, egg………………………100±20% |
| Precision (CV %): | Intra-lab assay: CV%≤10% |
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 5 vials of standard | 1mL each |
| High concentration standard 1ppm | 1mL |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Concentrated Sample Diluting Buffer (4×) | 40mL |
| IFU | 1 |
| Antigen coated plate | 12 test strips of 8 wells each |
| 5 vials of standard | 1mL each |
| High concentration standard 1ppm | 1mL |
Note:The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb and 5.4ppb.
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Homogenizer
—- Oscillator
—- Vortex
—- Centrifuge
—- Rotary evaporator/nitrogen evaporator
—- Balance (sensibility: 0.01g)
—- Transfer pipette: 10mL
—- Aurilave
—- Centrifuge tube: 10mL, 50mL
—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
- Reagent:
—- Acetonitrile (AR)
—- Dichloromethane (AR)
—- N-hexane (AR)
—- Deionized water
—-Concentrated sulfuric acid(18mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
