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    Animal Tissues, Eggs Antibiotic Quinolones ELISA Test Kit

    Qualitative or quantitative analysis of quinolones in animal tissues, eggs, etc.

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    The quinolones are a family of synthetic broad-spectrum antibiotics. The majority of quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.

    Use Principles: 

    Quinolones ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The quinolones antigen is precoated on the wells. The precoated antigen compete the quinolones antibody (antibody solution) with quinolones in the sample, anti- quinolones antibody binds to the quinolones-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.ME07B
    AnalyteQuinolones
    Sample TypeAnimal tissues, eggs, etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityCiprofloxacin (CIF) 25%
    Enrofloxacin (ENR) 100%
    Nadifloxacin (NAD) 323%
    Enoxacin (ENO) 37%
    Lomefloxacin (LOM) 21%
    Norfloxacin (NOR) 258%
    Marbolxacin (MAR) 19%
    Pefloxacin (PEF) 135%
    Ofloxacin (OFL) 55%
    Danofloxain (DAN) 69%
    Orbifloxacin(ORB) 17%
    Sarafloxacin (SAR) 33%
    Oxolinic acid (OXO) 51%
    Flumequine (FLU) 143%
     Calculate ResultAnalyze the result by using the software
    Sensitivity 0.2 ppb
    Limit of detectionTissue, egg 0.4ppb
    Percent Recovery, %Tissues, egg………………………100±20%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    5 vials of standard1mL each
    High concentration standard 1ppm1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer (4×)40mL
    IFU1
    Antigen coated plate12 test strips of 8 wells each
    5 vials of standard1mL each
    High concentration standard 1ppm1mL

     Note:The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb and 5.4ppb.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Homogenizer

    ---- Oscillator

    ---- Vortex

    ---- Centrifuge

    ---- Rotary evaporator/nitrogen evaporator

    ---- Balance (sensibility: 0.01g)

    ---- Transfer pipette: 10mL

    ---- Aurilave

    ---- Centrifuge tube: 10mL, 50mL

    ---- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    1. Reagent:

    ---- Acetonitrile (AR)

    ---- Dichloromethane (AR)

    ---- N-hexane (AR)

    ---- Deionized water

    ----Concentrated sulfuric acid(18mol/L)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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