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Animal Tissues, Eggs Antibiotic Quinolones ELISA Test Kit

Qualitative or quantitative analysis of quinolones in animal tissues, eggs, etc.

Meizheng Bio-Tech Company Overview. PDF

Background:

The quinolones are a family of synthetic broad-spectrum antibiotics. The majority of quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.

Use Principles: 

Quinolones ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The quinolones antigen is precoated on the wells. The precoated antigen compete the quinolones antibody (antibody solution) with quinolones in the sample, anti- quinolones antibody binds to the quinolones-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

ManufacturerMeizheng
Order No.ME07B
AnalyteQuinolones
Sample TypeAnimal tissues, eggs, etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityCiprofloxacin (CIF) 25%
Enrofloxacin (ENR) 100%
Nadifloxacin (NAD) 323%
Enoxacin (ENO) 37%
Lomefloxacin (LOM) 21%
Norfloxacin (NOR) 258%
Marbolxacin (MAR) 19%
Pefloxacin (PEF) 135%
Ofloxacin (OFL) 55%
Danofloxain (DAN) 69%
Orbifloxacin(ORB) 17%
Sarafloxacin (SAR) 33%
Oxolinic acid (OXO) 51%
Flumequine (FLU) 143%
 Calculate ResultAnalyze the result by using the software
Sensitivity 0.2 ppb
Limit of detectionTissue, egg 0.4ppb
Percent Recovery, %Tissues, egg………………………100±20%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 test strips of 8 wells each
5 vials of standard1mL each
High concentration standard 1ppm1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer (4×)40mL
IFU1
Antigen coated plate12 test strips of 8 wells each
5 vials of standard1mL each
High concentration standard 1ppm1mL

 Note:The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb and 5.4ppb.

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Homogenizer

—- Oscillator

—- Vortex

—- Centrifuge

—- Rotary evaporator/nitrogen evaporator

—- Balance (sensibility: 0.01g)

—- Transfer pipette: 10mL

—- Aurilave

—- Centrifuge tube: 10mL, 50mL

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

  1. Reagent:

—- Acetonitrile (AR)

—- Dichloromethane (AR)

—- N-hexane (AR)

—- Deionized water

—-Concentrated sulfuric acid(18mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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