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Feed Mycotoxin Deoxynivalenol (DON) ELISA Test Kit

The Deoxynivalenol ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Deoxynivalenol in feed and feed material.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF


Deoxynivalenol is known as vomitoxin or DON. It is a potent fungal toxin to affect crops grown in cool and moist conditions. Fusarium graminearum is the principal DON-producing fungus in grains but Fusarium culmorum is often involved as well especially in certain geographical areas of the world. Deoxynivalenol is a known immunosuppressant and may cause kidney problems. Consuming DON-contaminated grain can cause vomiting in humans and animals. Deoxynivalenol is formed by the Fusarium species and mainly affects corn, wheat, oats and barley.

Order No.HEM1896
Sample TypeFeed and feed material.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityDON………………………………………………100%
Oxynivalenol ……………………………………<1%
Result InterpretationAnalyze the result by using the software
Limit of quantitation250 ppb
Limit of detection200ppb
Percent Recovery  100±25%
Intra-lab assayCV%≤10%

Provided Materials and Reagent:

Antigen coated plate12 ×8 wells
5 vials of Standard1mL each
DON Enzyme Conjugate12mL
DON Antibody Solution12mL
Concentrated Wash Buffer (10X)40mL
Package Insert1
Plate Cover Sheet2
Testing Report1

Note: The concentrations of the standards are 0µg/kg, 200µg/kg, 700µg/kg, 2100µg/kg and 7000µg/kg respectively (the real concentration of the standards is 1/25 of the set concentrations).

Materials Required But Not Provided:

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Oscillator/ Vortex

—- Thermostat incubator ( If Room temperature can reach 25℃, it is not needed.)

—- Centrifuge (or quantitative filter)

—- Balance (sensibility: 0.01g)

—- Pipette: 50mL

—- Triangular flask (with stopper): 100mL

—- Centrifuge tube: 7mL

—- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL) Multi-channel pipet (30µL-300µL)

  1. Reagent:

—- Methanol (AR)

—- Deionized water

—- 18M concentrated sulfuric acid (H2SO4)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.


1-10, 11-100, 101-1000, 1001-10000


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