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    Feed Mycotoxin Deoxynivalenol (DON) ELISA Test Kit

    The Deoxynivalenol ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Deoxynivalenol in feed and feed material.

    Brochure-Grains & Feed Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Deoxynivalenol is known as vomitoxin or DON. It is a potent fungal toxin to affect crops grown in cool and moist conditions. Fusarium graminearum is the principal DON-producing fungus in grains but Fusarium culmorum is often involved as well especially in certain geographical areas of the world. Deoxynivalenol is a known immunosuppressant and may cause kidney problems. Consuming DON-contaminated grain can cause vomiting in humans and animals. Deoxynivalenol is formed by the Fusarium species and mainly affects corn, wheat, oats and barley.

    ManufacturerMeizheng
    TrademarkToxinFast®
    Order No.HEM1896
    AnalyteDeoxynivalenol
    Sample TypeFeed and feed material.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityDON………………………………………………100%
    3-AcDON…………………………………………<70%
    15-AcDON…………………………………….…<1%
    Oxynivalenol ……………………………………<1%
    Result InterpretationAnalyze the result by using the software
    Limit of quantitation250 ppb
    Limit of detection200ppb
    Percent Recovery  100±25%
    Intra-lab assayCV%≤10%

    Provided Materials and Reagent:

    Component96wells
    Antigen coated plate12 ×8 wells
    5 vials of Standard1mL each
    DON Enzyme Conjugate12mL
    DON Antibody Solution12mL
    Substrate10mL*2
    Concentrated Wash Buffer (10X)40mL
    Package Insert1
    Plate Cover Sheet2
    Testing Report1

    Note: The concentrations of the standards are 0µg/kg, 200µg/kg, 700µg/kg, 2100µg/kg and 7000µg/kg respectively (the real concentration of the standards is 1/25 of the set concentrations).

    Materials Required But Not Provided:

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Oscillator/ Vortex

    ---- Thermostat incubator ( If Room temperature can reach 25℃, it is not needed.)

    ---- Centrifuge (or quantitative filter)

    ---- Balance (sensibility: 0.01g)

    ---- Pipette: 50mL

    ---- Triangular flask (with stopper): 100mL

    ---- Centrifuge tube: 7mL

    ---- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL) Multi-channel pipet (30µL-300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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