Background:
Deoxynivalenol is known as vomitoxin or DON. It is a potent fungal toxin to affect crops grown in cool and moist conditions. Fusarium graminearum is the principal DON-producing fungus in grains but Fusarium culmorum is often involved as well especially in certain geographical areas of the world. Deoxynivalenol is a known immunosuppressant and may cause kidney problems. Consuming DON-contaminated grain can cause vomiting in humans and animals. Deoxynivalenol is formed by the Fusarium species and mainly affects corn, wheat, oats and barley.
| Manufacturer | Meizheng |
| Trademark | ToxinFast® |
| Order No. | HEM1896 |
| Analyte | Deoxynivalenol |
| Sample Type | Feed and feed material. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | DON………………………………………………100% 3-AcDON…………………………………………<70% 15-AcDON…………………………………….…<1% Oxynivalenol ……………………………………<1% |
| Result Interpretation | Analyze the result by using the software |
| Limit of quantitation | 250 ppb |
| Limit of detection | 200ppb |
| Percent Recovery | 100±25% |
| Intra-lab assay | CV%≤10% |
Provided Materials and Reagent:
| Component | 96wells |
| Antigen coated plate | 12 ×8 wells |
| 5 vials of Standard | 1mL each |
| DON Enzyme Conjugate | 12mL |
| DON Antibody Solution | 12mL |
| Substrate | 10mL*2 |
| Concentrated Wash Buffer (10X) | 40mL |
| Package Insert | 1 |
| Plate Cover Sheet | 2 |
| Testing Report | 1 |
Note: The concentrations of the standards are 0µg/kg, 200µg/kg, 700µg/kg, 2100µg/kg and 7000µg/kg respectively (the real concentration of the standards is 1/25 of the set concentrations).
Materials Required But Not Provided:
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Oscillator/ Vortex
—- Thermostat incubator ( If Room temperature can reach 25℃, it is not needed.)
—- Centrifuge (or quantitative filter)
—- Balance (sensibility: 0.01g)
—- Pipette: 50mL
—- Triangular flask (with stopper): 100mL
—- Centrifuge tube: 7mL
—- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL) Multi-channel pipet (30µL-300µL)
- Reagent:
—- Methanol (AR)
—- Deionized water
—- 18M concentrated sulfuric acid (H2SO4)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
