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    Furaltadone ELISA Test Kit for Poultry Tissues, Liver and Eggs

    The Furaltadone (AMOZ) ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of Furaltadone (AMOZ) in samples of poultry tissues, liver, eggs etc.

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

    Use Principles: 

    Furaltadone (AMOZ) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The AMOZ antigen is precoated on the wells. The precoated antigen compete the AMOZ antibody (antibody solution) with AMOZ in the sample, anti-AMOZ antibody binds to the AMOZ-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the AMOZ concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.ME04A
    AnalyteFuraltadone (AMOZ)
    Sample TypePoultry tissues, liver, eggs etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityFuraltadone (AMOZ)….....................…100%
    Furazolidone (AOZ)…………………...<0.1%
    Furantoin (AHD)……………….....……<0.1%
    Furacilin (SEM)…………………………<0.1%
     Calculate ResultAnalyze the result by using the software
    Sensitivity 0.05ppb
    Limit of detection0.1ppb
    Percent Recovery, %Tissues, liver, egg………………………100±30%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of standard1mL each
    High concentrated standard (100ppb)1mL
    AMOZ Enzyme Conjugate6mL
    AMOZ Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated wash buffer (10×)40mL
    Concentrated sample diluting buffer (2X)40mL
    Concentrated derivatization reagent (10X)1mL
    IFU1
    Testing Report1
    Plate cover sheet2

     Note:The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex mixer

    ---- Centrifuge

    ---- Homogenizer

    ---- Constant temperature incubator

    ---- Balance (sensibility: 0.01g)

    ---- Nitrogen evaporator

    ---- Water bath

    ---- Transfer pipette: 10mL

    ---- Centrifuge tube: 10mL, 50mL,7mL

    ---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Ethyl acetate (AR)

    ---- Deionized water

    ---- N-hexane (AR)

    ---- Methanol (AR)

    ---- Concentrated hydrochloric acid (AR)

    ---- NaOH (AR)

    ---- Dipotassium Hydrogen Phosphate Trihydrate (K2HPO4.3H2O) (AR)

    ---- Concentrated sulfuric acid (18 mol/L)

     Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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