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Meat Veterinary Drugs Residues Olaquindox Metabolite ELISA Test Kit

Order No.: ME15AER

LOD: 0.1 ppb

Package: 96T

[Scope of Application]

Qualitative or quantitative analysis of Olaquindox Metabolite  in animal tissue (pork, chicken, fish, shrimp, duck, beef, lamb).

[Summary]

Olaquindox is a quinoxaline drug, a synthetic broad-spectrum antibiotic. Studies have shown that olaquindox and its deoxymetabolites have carcinogenicity, teratogenicity and photosensitivity toxicity.

The Olaquindox Metabolite ELISA Test Kit produced by Meizheng is fast, easy-to-use, accurate and has high sensitivity, with 45 minutes operation time, which can minimize the operating error and working intensity.

[Principles]

Olaquindox Metabolite ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Olaquindox Metabolite antigen is precoated on the wells. The precoated antigen compete  the Olaquindox Metabolite antibody (Antibody Solution) with Olaquindox Metabolite in the sample, Olaquindox Metabolite antibody binds to the Olaquindox Metabolite Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Olaquindox Metabolite contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Olaquindox Metabolite in the sample  can be calculated.

[Cross Reactivity]

Olaquindox Metabolite:100%

2-Quinoxalinecarboxylic Acid: 60%

[Materials Required But Not Provided]

  1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Vortex

—- Constant Temperature Incubator (Optional)

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Termovap Sample Concentrator

—- Centrifuge tube: 5mL/7mL, 50mL

—- Graduated Pipette: 10mL

—- Rubber Bulb Syringe

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

  1. Reagent:

—- Deionized Water

—- Ethyl Acetate (AR)

—- N-hexane (AR)

[Provided Materials and Reagent]

Component96 wells
Antigen Coated Plate96T
5 vials of Standard1mL each
High Concentration Standard (100ppb)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standards are 0ppb, 0.1ppb, 0.3ppb, 0.9ppb,2.7ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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