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    Nitroimidazole ELISA Test Kit for Animal Tissues and Eggs

    The Nitroimidazole ELISA Test Kit is competitive ELISA for the qualitative or quantitative analysis of Nitroimidazole in samples of animal tissues, eggs etc.

    Order No.: ME33AER

    Summary

    Nitroimidazole antibacterial drugs are mainly used to treat and prevent infections caused by trichomoniasis, amoeba protozoa and anaerobic bacteria. However, due to the carcinogenic effects of these drugs, many countries prohibit their use in the breeding of livestock, poultry and aquatic products.

    Principles

    The Nitroimidazole ELISA Test kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Nitroimidazole in the sample, anti-Nitroimidazole antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Nitroimidazole concentration of the samples is derived. 

    Cross-reactivity 

    Metronidazole…………………………………………………………………………100%

    Dimetridazole…………………………………………………………………………100%

    Hydroxymethyl metronidazole…………………………………………………20%

    Hydroxy metronidazole……………………………………………………………15%

    Secnidazole, ornidazole……………………………………………………………15%

    Ronidazole…………………………………………………………………………………2%

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Homogenizer

    ---- Vortex

    ---- Centrifuge

    ---- Termovap Sample Concentrator

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Rubber Bulb Syringe

    ---- Constant temperature incubator (Optional)

    ---- Centrifuge tube (5mL/7mL, 50mL)

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- Sodium hydroxide (NaOH) (AR)

    ---- Ethyl acetate (AR)

    ---- N-hexane (AR)

    ---- 18M concentrated sulfuric acid (H2SO4)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate96 T
    6 vials of Standard2mL each
    High concentration standard (100ppb)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated sample diluting buffer (2×)50mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb, respectively.

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