Order No.: ME04DER
LOD: 0.05 ppb
Sample: Meat, Seafood, Bullfrog
Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.
Furaltadone Metabolite ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Furaltadone Metabolite antigen is precoated on the wells. The precoated antigen compete the Furaltadone Metabolite antibody (Antibody Solution) with Furaltadone Metabolite in the sample, Furaltadone Metabolite antibody binds to the Furaltadone Metabolite Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Furaltadone Metabolite contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Furaltadone Metabolite in the sample can be calculated.
Furaltadone Metabolite 100%
Furazolidone Metabolite <0.1%
Furantoin Metabolite <0.1%
Furacilin Metabolite <0.1%
Materials Required But Not Provided
---- Microplate Reader (450nm/630nm filter)
---- Constant Temperature Incubator
---- Balance (sensibility: 0.01g)
---- Termovap Sample Concentrator
---- Water Bath
---- Graduated Pipette: 10mL
---- Rubber Bulb Syringe
---- Centrifuge Tube: 10mL, 50mL,7mL
---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
---- Ethyl acetate (AR)
---- Deionized water
---- N-hexane (AR)
---- Methanol (AR)
---- Concentrated hydrochloric acid (HCl) (AR)
---- Sodium hydroxide (NaOH) (AR)
---- Dipotassium hydrogen phosphate trihydrate (K2HPO4‧3H2O) (AR)
---- 18M concentrated sulfuric acid (H2SO4)
[Provided Materials and Reagent]
|Antigen Coated Plate||96T|
|6 vials of Standard||1mL each|
|High Concentration Standard (100ppb)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer (2×)||40mL|
|Concentrated Derivatization Reagent (10×)||1mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.