Qualitative or Quantitative Antibiotic Quinolones ELISA Test Kit

【Summary】

The quinolones are a family of synthetic broad-spectrum antibiotics. Most quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.

【Principles】

The Quinolones ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

【Cross Reactivity】

Enrofloxacin (ENR) 100%

Ciprofloxacin (CIP) ＞100%

Norfloxacin (NOR) ＞100%

Ofloxacin (OFL) 43%

Sarafloxacin (SAR) 36%

Pefloxacin (PEF) ＞100%

Lomefloxacin (LOM) 46%

Danofloxain (DAN) ＞100%

Enoxacin (ENO) ＞100%

Flumequine (FLU) 57%

Nadifloxacin (NAD) 37%

Oxolinic acid (OXO) ＞100%

Orbifloxacin(ORB) 23%

Marbolxacin (MAR) 23%

Difloxacin (DIF) 54%

【Materials Required But Not Provided】

1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Vortex

—- Constant Temperature Incubator

—- Centrifuge

—- Homogenizer

—- Balance (sensibility: 0.01g)

—- Rubber Bulb Syringe

—- Graduated pipette: 10mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

2. Reagent:

—- Deionized water

—- Methanol

【Provided Materials and Reagent】

Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, and 4.05ppb.