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Qualitative or Quantitative Antibiotic Quinolones ELISA Test Kit

The Quinolones ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of quinolones in samples of animal tissues, eggs, egg powder, swine urine etc.

Order No.: ME07CERV

Summary

The quinolones are a family of synthetic broad-spectrum antibiotics. Most quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.

Principles

The Quinolones ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

Cross Reactivity

Enrofloxacin (ENR) 100%

Ciprofloxacin (CIP) >100%

Norfloxacin (NOR) >100%

Ofloxacin (OFL) 43%

Sarafloxacin (SAR) 36%

Pefloxacin (PEF) >100%

Lomefloxacin (LOM) 46%

Danofloxain (DAN) >100%

Enoxacin (ENO) >100%

Flumequine (FLU) 57%

Nadifloxacin (NAD) 37%

Oxolinic acid (OXO) >100%

Orbifloxacin(ORB) 23%

Marbolxacin (MAR) 23%

Difloxacin (DIF) 54%

Materials Required But Not Provided

  1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Vortex

—- Constant Temperature Incubator

—- Centrifuge

—- Homogenizer

—- Balance (sensibility: 0.01g)

—- Rubber Bulb Syringe

—- Graduated pipette: 10mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

2. Reagent:

—- Deionized water

—- Methanol

Provided Materials and Reagent

Component96 wells
Antigen coated plate96T
6 vials of Standard1mL each
High concentration standard (1ppm)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Stop Solution6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, and 4.05ppb.

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