Search

In Stock

Qualitative or Quantitative Antibiotic Quinolones ELISA Test Kit

The Quinolones ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of quinolones in samples of animal tissues, eggs, egg powder, swine urine etc.

Order No.: ME07CERV

Summary

The quinolones are a family of synthetic broad-spectrum antibiotics. Most quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.

Principles

The Quinolones ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

Cross Reactivity

Enrofloxacin (ENR) 100%

Ciprofloxacin (CIP) >100%

Norfloxacin (NOR) >100%

Ofloxacin (OFL) 43%

Sarafloxacin (SAR) 36%

Pefloxacin (PEF) >100%

Lomefloxacin (LOM) 46%

Danofloxain (DAN) >100%

Enoxacin (ENO) >100%

Flumequine (FLU) 57%

Nadifloxacin (NAD) 37%

Oxolinic acid (OXO) >100%

Orbifloxacin(ORB) 23%

Marbolxacin (MAR) 23%

Difloxacin (DIF) 54%

Materials Required But Not Provided

  1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Vortex

—- Constant Temperature Incubator

—- Centrifuge

—- Homogenizer

—- Balance (sensibility: 0.01g)

—- Rubber Bulb Syringe

—- Graduated pipette: 10mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

2. Reagent:

—- Deionized water

—- Methanol

Provided Materials and Reagent

Component96 wells
Antigen coated plate96T
6 vials of Standard1mL each
High concentration standard (1ppm)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Stop Solution6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, and 4.05ppb.

Bestsellers

Aflatoxin B1 Quantitative Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin B1 and is intended for use in nuts, grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

Immunoaffinity Column for 2-in-1 Aflatoxins (B1,B2,G1,G2) and Ochratoxin A

The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample  solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.Order No.: HCM3725B/HCM3750BProduct Properties: AFT B1/B2/G1/G2 300ng, OTA 100ngSpecifications: 3mL,25T/50T

Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Aflatoxin M1 Immunoaffinity Column

Order No.: QC0068,QC0069,QC0131,QC0071Column Capacity:100 ngVolumn: 3mLPackage: 25/50T 

Milk Tylosin and Tilmicosin Rapid Test Kit

Tylosin/Tilmicosin Rapid Test Kit is a Lateral Flow assay that determines the residues for the presence of tylosin/tilmicosin residues in raw milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Food Mycotoxin Deoxynivalenol (DON) ELISA Test Kit

The Deoxynivalenol ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Deoxynivalenol in wheat, wheat flour, bran and wheat middlings, etc.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

 

Back to Top
Product has been added to your cart