Summary
Tilmicosin is a macrolide antibiotic mainly used to prevent livestock and poultry pathogens and mycoplasma infections.
Order No.: ME41AER
LOD: 0.5 ppb
Package: 96T
Use Principles
Tilmicosin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Tilmicosin antigen is precoated on the wells. The precoated antigen compete the Tilmicosin antibody (antibody solution) with Tilmicosin in the sample, anti-Tilmicosin antibody binds to the Tilmicosin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Tilmicosin concentration of the samples is derived.
Cross-reactivity
Tilmicosin ………………………………………100%
Tylosin ……………………………………………<1%
Spectinomycin, lincomycin……………<0.1%
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Homogenizer
—- Oscillator
—- Vortex
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Transfer pipette: 10mL
—- Aurilave
—- Centrifuge tube: 5mL, 7mL, 50mL
—-Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30µL -300µL)
- Reagent:
—- Deionized water
—- Concentrated sulfuric acid (18 mol/L)
Provided Materials and Reagent
Component | 96 wells |
Antigen coated plate | 12 test strips of 8 wells each |
6 vials of standard | 1mL each |
Enzyme Conjugate | 6mL |
Antibody Solution | 6mL |
Substrate A | 6mL |
Substrate B | 6mL |
Concentrated Wash Buffer (10×) | 40mL |
Concentrated sample diluting buffer | 40mL |
Package Insert | 1 |
Testing Report | 1 |
Plate Cover Sheet | 2 |
Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb and 40.5ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.