Animal Tissue, Seafood and Egg Antibiotic Tetracyclines ELISA Test Kit

Summary

Tetracyclines are antibiotics which inhibit the bacterial growth by stopping protein synthesis. They have been widely used for the past forty years as therapeutic agent in human and veterinary medicine but also as growth promoter in animal husbandry. But the abuse of antibiotics will lead to the residues in the livestock and indirectly affect the health of human being.

HPLC method is now widely used in the detection of tetracyclines residues, but its high cost and complex operation make it unacceptable.

Order No.: ME09AER

LOD: 0.2 ppb

Package: 96T

Use Principles

Tetracyclines (TCs) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The tetracycline antigen is precoated on the wells. The precoated antigen compete the tetracycline antibody (antibody solution) with tetracycline in the sample, anti- tetracycline antibody binds to the tetracycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived.

Cross-reactivity

Tetracycline…………………………………………………100%

Doxycycline…………………………………………………90%

Chlortetracycline…………………………………………180%

Oxytetracycline……………………………………………70%

Materials Required But Not Provided

1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Homogenizer

—- Oscillator

—- Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Transfer pipette: 10mL

—- Aurilave

—- Centrifuge tube: 5mL/7mL, 50mL

—- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)

Multi-channel pipette (300µL)

2. Reagent:

—- Deionized water

—- Methanol (AR)

—- Concentrated sulfuric acid (18mol/L)

Provided Materials and Reagent

Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.