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    Animal Tissue Veterinary Drugs Residues Furantoin Metabolite ELISA Test Kits

    Qualitative or quantitative analysis of Furantoin Metabolite in animal tissue (chicken, pork, fish, shrimp, duck, bullfrog).

    Order No.: ME06DER

    LOD: 0.05 ppb

    Package: 96T

    Summary

    Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

    Use Principles

    Furantoin Metabolite ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Furantoin Metabolite antigen is precoated on the wells. The precoated antigen compete the Furantoin Metabolite antibody (Antibody Solution) with Furantoin Metabolite in the sample, Furantoin Metabolite antibody binds to the Furantoin Metabolite Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Furantoin Metabolite contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Furantoin Metabolite in the sample can be calculated.

    Cross-reactivity

    Furantoin Metabolite 100%

    Furaltadone Metabolite <0.1%

    Furacilin Metabolite <0.1%

    Furazolidone Metabolite <0.1%

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Oscillator

    ---- Vortex

    ---- Centrifuge

    ---- Homogenizer

    ---- Constant Temperature Incubator

    ---- Water Bath

    ---- Termovap Sample Concentrator

    ---- Balance (sensibility: 0.01g)

    ---- Graduated Pipette: 10mL

    ---- Rubber Bulb Syringe

    ---- Centrifuge Tube: 10mL or 7mL, 50mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized Water

    ---- Ethyl Acetate (AR)

    ---- N-hexane (AR)

    ---- Methanol (AR)

    ---- Concentrated hydrochloric acid (HCl) (AR)

    ---- Sodium hydroxide (NaOH) (AR)

    ---- Dipotassium hydrogen phosphate trihydrate (K2HPO4·3H2O) (AR)

    ---- 18M concentrated sulfuric acid (H2SO4)

    [Provided Materials and Reagent]

    Component96 wells
    Antigen Coated Plate96T
    6 vials of Standard1mL each
    High Concentration Standard (100ppb)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer (2×)40mL
    Concentrated Derivatization Reagent (10×)1mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb,4.05ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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